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| Introduction | Batroxobin is a serin protease that reduces fibronogen levels and is originally extracted from snake venom of Bothrops Atrox. Batroxobin is used in defibrinogenation and thrombolysis and also has an effect on c-fos gene and growth factor.Batroxobin can efficiently restrain proliferation of VSMCs, by blocking the release and uptake of Ca2+, thus influencing [Ca2+]i.Batroxobin is a single chain glycopeptide with a molecular mass of 43kDa on SDS-PAGE gel and its pI-6.6. Batroxobin converts fibrinogen to fibrin throµgh the restricted release of fibrinopeptide-A from fibrinogen to promote blood to clot. Unlike thrombin, it is not affected by heparin and hirudin. |
| Synonyms | Thrombin-like enzyme batroxobin, EC 3.4.21.74, BX, Bothrops atrox serine proteinase, Venombin-A, Defibrase, Reptilase, Batroxobin. |
| Source | Pichia Pastoris. |
| Physical Appearance | Sterile Filtered white lyophilized powder. |
| Formulation | The Batroxobin protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM sodium acetate buffer, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Batroxobin in sterile 18M-cm H2O not less than 100 |
| Stability | Batroxobin althoµgh stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino Acid Sequence | VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCAEPRKPAFYTK VFDYLPWIQS IIAGNKTATC P. |
| Purity | Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Biological Activity | Batroxobin’s biological activity was found to be of no less than 500KU/mg. (Klobusitzky Unit). |
| Usage | NeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals. |
| References | Title:Impaired clot retraction in factor XIII A subunit-deficient mice Supplemental materials for: Kasahara et alPublication:Published online before print December 8, 2009, doi: 10.1182/blood-2009-06-227645 Blood December 8, 2009 Link:http://bloodjournal.hematologylibrary.org/content/115/6/1277/suppl/DC1 |
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