CD274 Human Recombinant produced in E. coli is a single polypeptide chain containing 245 amino acids (19-238) and having a molecular mass of 27.9kDa (Molecular weight on SDS-PAGE will appear higher).CD274 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CD274 takes part in the costimulatory signal, vital for T-cell proliferation and creation of IL10 and IFNG, in a PDCD1-independent and an IL2-dependent manner. CD274 collaboration with PDCD1 inhibits cytokine production and T-cell proliferation.
The CD274 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.