CDC25A Human-Cell Division Cycle

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CDC25A Human

Qty


Total
$65
Catalog #
ENPS-098
Size
Description
CDC25A Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 560 amino acids (1-524 a.a.) and having a molecular mass of 63.2kDa. The CDC25A is purified by proprietary chromatographic techniques.
Additional Information
IntroductionM-phase inducer phosphatase 1 (CDC25A) belongs to the CDC25 family of phosphatases. CDC25A is essential for progression from G1 to the S phase of the cell cycle. CDC25A activates the cyclin-dependent kinase CDC2 by eliminating 2 phosphate groups. CDC25A is specifically degraded in reaction to DNA damage, which inhibits cells with chromosomal abnormalities from progressing in the course of cell division. CDC25A is an oncogene, thoµgh its exact function in oncogenesis has not been determined.
SynonymsM-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A, CDC25A2.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe CDC25A solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 20% glycerol, 0.2M NaCl and 1mM EDTA.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMRGSHHHHHH
GMASMTGGQQ
MGRDLYDDDD
KDRWGSMELG
PEPPHRRRLL
FACSPPPASQ
PVVKALFGAS
AAGGLSPVTN
LTVTMDQLQG
LGSDYEQPLE
VKNNSNLQRM
GSSESTDSGF
CLDSPGPLDS
KENLENPMRR
IHSLPQKLLG
CSPALKRSHS
DSLDHDIFQL
IDPDENKENE
AFEFKKPVRP
VSRGCLHSHG
LQEGKDLFTQ
RQNSAPARML
SSNERDSSEP
GN
PurityGreater than 80.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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