CDC34 Human-Cell Division Cycle

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CDC34 Human

Qty


Total
$65
Catalog #
ENPS-362
Size
Description
CDC34 Human Recombinant protein fused to a 20 amino acid N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 256 amino acids (1-236 a.a.) and having a molecular mass of 28.9 kDa. The CDC34 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionCDC34 is important in the control of cell cycle and DNA replication. Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. Cdc34 substrates that have been characterized include I?B, B-Myb, Wee1, MyoD, ICERII?, ATF5, p27Xic1, and p27Kip1. Additionally, substrates such as ?-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. Cdc34 has been demonstrated to self-associate throµgh a domain in the C-terminus, and is phosphorylated and ubiquitinylated in vivo. This protein is useful for in vitro ubiquitinylation reactions.
SynonymsUB2R1, CDC-34, Ubiquitin-conjµgating enzyme E2 R1, Ubiquitin-protein ligase R1, Ubiquitin-conjµgating enzyme E2-32 kDa complementing, E2-CDC34, CDC34, EC 6.3.2.19, UBC3, UBE2R1.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe CDC34 (1mg/ml) protein solution contains 20mM Tris-Hcl pH-8, 10% glycerol, 50mM NaCl and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MARPLVPSSQ
KALLLELKGL
QEEPVEGFRV
TLVDEGDLYN
WEVAIFGPPN
TYYEGGYFKA
RLKFPIDYPY
SPPAFRFLTK
MWHPNIYETG
DVCISILHPP
VDDPQSGELP
SERWNPTQNV
RTILLSVISL
LNEPNTFSPA
NVDASVMYRK
WKESKGKDRE
YTDIIRKQVL
GTKVDAERDG
VKVPTTLAEY
CVKTKAPAPD
EGSDLFYDDY
YE
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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