Collagen Human-Collagen

our services
our products
Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1-888.733.6849
+1-617.299.7367 (Int’l)
+44-203.695.3777 (UK)

Fax:

+1-888.733.6849
+1-617.299.7367 (Int’l)

Our customer service representatives are available 24 hours, Monday through Friday to assist you.

Collagen Human

Qty


Total
$279.5
Catalog #
PRPS-366
Size
Description
Collagen Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain a molecular mass of 96,000 Dalton containing human collagen’s cDNA transcribed reversely from mRNA.The Collagen is purified by proprietary chromatographic techniques.
Additional Information
IntroductionCollagen remained the initial functions of collagen and had some new characters because of its structure, such as reversible colloid with heat and easy processed without molecular reduction and so on. However, animal collagen extracted from animal tissues was insoluble except for the molecular weight reduction. The fetal shortcomings of animal collagen restricted the application is its harmful virus, but Human-like Collagen made up these drawbacks. Collagen (extracted from animal skin, tendons and hides) is potential risk in virus with the potential to cause side effect and the potential for the contamination of bovine collagen with the fatal mad cow disease. Collagen dissolves in water, which is another advantage to compete with animal collagen. It is different from hydrolyzed collagen produced by hydrolyzing animal collagen to short peptide linkage with a lower molecular weight. Human-like collagen was expressed by human collagen gene in host, so mmunogenicity could be lessened much by compared with animal collagen after it entered into human body.
SynonymsNULL
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe protein was lyophilized after from a sterile solution containing no additives.
SolubilityIt is recommended to reconstitute the lyophilized Collagen in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Collagen althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Collagen should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceThe
sequence
of
the
first
fifteen
N-terminal
amino
acids
was
determined
and
was
found
to
be
MDPVVLQRRDWENPG.Amino
acid
%Asp
0.39;
Thr
0.16;
Ser
5.70;
Pro
18.87;
Gly
29.17;
Ala
13.38;
Val
.71;
Met
.18;
Ile
0.16;
Leu
8.04;
Tyr
0.90;
Phe
0.15;
Lys
0.27;
His
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological Activity1. Cultivation of BHK-21 and 2BS cell line BHK-21 or 2BS cells were plated onto the bottom of the 96-well cell culture plates at the density of 1*103cells/well, respectively, with 0.1ml RPMI1640 contained 10% fetal bovine serum in the well. BHK-21 or 2BS cells were incubated in standard conditions (at 37°C with 5% CO2 in a humidified incubator) for 24 hours. 2. Biochemical activity analysis of Human-like Collagen Complete culture media was removed after 24 hours, Collagen was added at various concentrations 0.01%,0.03%,0.05%,0.07%,0.09%,0.10%,0.11%,0.13% and a complete medium was used as blank control. Cells were incubated in standard conditions (at 37°C with 5% CO2 in a humidified incubator). The morphology of cell was observed by reverse microscope after 24hr, 48hr, 72hr, 96hr, and 120hr. Thereafter, the viable cell count was determined by a colorimetric method using MTT (3-4,5dimethylthiazol-2yl-2, 5 diphenyl tetrazolium bromide) after 24hr, 48hr, 72hr, 96hr and 120hr. A490 was evaluated by ELISA reader. 3. Results The results demonstrate that Collagen has no significant effect on cell morphology. MTT analysis shows that Collagen has remarkable effect on cell proliferation when its concentration is higher than 0.03%. Cell proliferation was improved by 40% to 60% compared to control.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
ReferencesTitle:Radiographic Evaluation of Pulpal and Periapical Response of Dogs’ Teeth After Pulpotomy and Use of Recombinant Human Bone Morphogenetic Protein-7 as a Capping Agent.Publication:Journal of Dentistry for Children-75:1, 2008 Radiographic Evaluation after Pulpotomy using rHuBMP-7 da Silva et al.Link:http://www.traducoesemsaude.com/publicacoes/files/Francisco%20BMP%20JCD_2008.pdf
Background

N/A

Protocol

N/A

MSDS
Reviews
Neo Scientific welcomes feedback from its customers.

If you have used an our product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information.

If you have any additional inquiries please email technical services at [email protected].

Thank you for your support.

promotions

Image

Custom Monoclonal Antibody
MassAb Technologies
Image
Free Trial Custom Polyclonal Antibody
You will receive free purified antibodies for validation
Image
Custom Peptides Synthesis

Start from $40/each

new technologies

Image
Optimized for enhanced
expression levels
XPromoter 2.0
Image
E.coli/insect cells/293 & CHO

3 in 1 expression system
Image
MassAb Technologies

Cover All Epitopes