Cys-Protein G His Tag Recombinant produced in E.Coli is a 202 amino acids protein which contains amino acid 190-384 of the Streptococcus sp with a 6-His tag Cysteine residue at the C-terminus, has a predicted molecular mass of approximately 22.5 kDa. But it migrates with an apparent molecular mass of 32kDa in SDS-PAGE.The Protein G His Tag is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized with no additive.
It is recommended to reconstitute the lyophilized Protein G in sterile 18M?-cm H2O not less than 100
Lyophilized Protein G althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
NeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a.2. Does not bind to human IgM, IgD and IgA.