DCTD Human-Deaminase

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DCTD Human

Qty


Total
$65
Catalog #
ENPS-545
Size
Description
DCTD Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Additional Information
IntroductionDCTD is an allosteric enzyme that exists as a homohexamer and is part of the cytidine and deoxycytidylate deaminase protein family. DTCD useszinc as a cofactor to catalyze the deamination of dCMP to dUMP, thus making the nucleotide substrate (dUMP) that is used by thymidylate synthase.
SynonymsDeoxycytidylate deaminase, EC 3.5.4.12, dCMP Deaminase, DCTD, MGC111062.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe DCTD solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA & 20% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MSEVSCKKRD
DYLEWPEYFM
AVAFLSAQRS
KDPNSQVGAC
IVNSENKIVG
IGYNGMPNGC
SDDVLPWRRT
AENKLDTKYP
YVCHAELNAI
MNKNSTDVKG
CSMYVALFPC
NECAKLIIQA
GIKEVIFMSD
KYHDSDEATA
ARLLFNMAGV
TFRKFIPKCS
KIVIDFDSIN
SRPSQKLQ.
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished forLABORATORY RESEARCHUSEONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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