DnaK Lid Covering Substrate E.Coli-Heat Shock Protein

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DnaK Lid Covering Substrate E.Coli

Qty


Total
$65
Catalog #
HYPS-018
Size
Description
Recombinant DnaK Lid Covering Substrate domain produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 14.6 kDa.
Additional Information
IntroductionDnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. DnaK(amino acid 508-638) was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
SynonymsHSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMNEDEIQKMV
RDAEANAEAD
RKFEELVQTR
NQGDHLLHST
RKQVEEAGDK
LPADDKTAIESALTALETAL
KGEDKAAIEA
KMQELAQVSQ
KLMEIAQQQH
AQQQTAGADASANNAKDDDVVDAEFEEVKDKK.
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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