Dnak SBD C-terminus E.Coli-Heat Shock Protein

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Dnak SBD C-terminus E.Coli

Qty


Total
$65
Catalog #
HYPS-016
Size
Description
Recombinant Dnak Substrate Binding Domain C-terminal produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids and having a molecular mass of 27.7 kDa.
Additional Information
IntroductionDnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.The protein coding region of the substrate binding domain of DnaK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
SynonymsHSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMDVKDVLLLD
VTPLSLGIET
MGGVMTTLIA
KNTTIPTKHS
QVFSTAEDNQ
SAVTIHVLQGERKRAADNKS
LGQFNLDGIN
PAPRGMPQIE
VTFDIDADGI
LHVSAKDKNS
GKEQKITIKA
SSGLNEDEIQ
KMVRDAEANA
EADRKFEELV
QTRNQGDHLL
HSTRKQVEEA
GDKLPADDKTAIESALTALE
TALKGEDKAA
IEAKMQELAQ
VSQKLMEIAQ
QQHAQQQTAG
ADAS
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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