EPGN Human-Others

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EPGN Human

Qty


Total
$65
Catalog #
CYPS-608
Size
Description
Epigen Recombinant Human produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques.
Additional Information
IntroductionEPGN is an EGF-related polypeptide growth factor that signals throµgh the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence.
SynonymsEPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationEPGN was lyophilized from 20mM PBS buffer pH-7.4 containing 130mM sodium chloride.
SolubilityIt is recommended to reconstitute the lyophilized Epigen in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Epigen althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPGN should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceAVTVTPPITA
QQADNIEGPI
ALKFSHLCLE
DHNSYCINGA
CAFHHELEKA
ICRCFTGYTG
ERCEHLTLTS
YA.
PurityGreater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED50 for this effect is 150-300ng/ml corresponding to a specific activity of 3,334-6,667IU/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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