FEN1 Human-Endonuclease

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FEN1 Human

Qty


Total
$65
Catalog #
ENPS-475
Size
Description
FEN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-380 a.a.) and having a molecular mass of 42.5 kDa. The FEN1 protein is purified by standard chromatogrpahy techniques.
Additional Information
IntroductionFEN1 removes 5'' overhanging flaps in DNA repair and processes the 5'' ends of Okazaki fragments in lagging strand DNA synthesis. The interaction between FEN1 and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, therefore passing the substrate from one enzyme to another. FEN1 is part of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5'' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
SynonymsFEN-1, MF1, RAD2, Maturation Factor-1, MF-1, Flap endonuclease 1, Flap structure-specific endonuclease 1, Maturation factor 1, hFEN-1, DNase IV, FEN1.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe protein contains 20mM Tris-HCl buffer pH-8.0, 1mM DTT, 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGIQGLAKLI
ADVAPSAIRE
NDIKSYFGRK
VAIDASMSIY
QFLIAVRQGG
DVLQNEEGET
TSHLMGMFYR
TIRMMENGIK
PVYVFDGKPP
QLKSGELAKR
SERRAEAEKQ
LQQAQAAGAE
QEVEKFTKRL
VKVTKQHNDE
CKHLLSLMGI
PYLDAPSEAE
ASCAALVKAG
KVYAAATEDM
DCLTFGSPVL
MRHLTASEAK
KLPIQEFHLS
RILQELGLNQ
EQFVDLCILL
GS
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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