FGF 2 Rat-Fibroblast Growth Factor

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FGF 2 Rat

Qty


Total
$65
Catalog #
CYPS-615
Size
Description
bFGF Rat Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16.3 kDa.The bFGF2 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionFGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.
SynonymsHBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationFGF-b was lyophilized from 1 mg/ml solution after extensive dialysis against 20mM phosphate buffer, pH 7.4 and 130mM NaCl.
SolubilityIt is recommended to reconstitute the lyophilized Rat bFGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Rat bFGF althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat FGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequencePALPEDGGGA
FPPGHFKDPK
RLYCKNGGFF
LRIHPDGRVD
GVREKSDPHV
KLQLQAEERG
VVSIKGVCAN
RYLAMKEDGR
LLASKCVTEE
CFFFERLESN
NYNTYRSRKY
SSWYVALKRT
GQYKLGSKTG
PGQKAILFLP
MSAKS.
PurityGreater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to a specific activity of 5,000,000 IU/mg.
UsageNeoScientific's products are furnished form LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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