FLT1 Human-VEGF Receptors

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FLT1 Human

Qty


Total
$65
Catalog #
PKPS-247
Size
Description
Soluble FLT1 Human Recombinant produced in baculovirus is monomeric, glycosylated, polypeptide containing 688 amino acids and having a molecular mass of 96 kDa. The soluble receptor protein contains only the first 6 extracellular domains, which contain all the information necessary for binding of VEGF.The FLT1 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionEndothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
SynonymsFLT-1, FLT1, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1, VEGFR-1.
SourceInsect Cells.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationFLT1 was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.
SolubilityIt is recommended to reconstitute the lyophilized FLT1 in sterile water not less than 100
StabilityLyophilized FLT-1 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FLT1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMVSYWDTGVL
LCALLSCLLL
TGSSSGSKLK
DPELSLKGTQ
HIMQAGQTLH
LQCRGEAAHK
WSLPEMVSKE
SERLSITKSA
CGRNGKQFCS
TLTLNTAQAN
HTGFYSCKYL
AVPTSKKKET
ESAIYIFISD
TGRPFVEMYS
EIPEIIHMTE
GRELVIPCRV
TSPNITVTLK
KFPLDTLIPD
GKRIIWDSRK
GFIISNATYK
EIGLLTCEAT
VNGHLYKTNY
LTHRQTNTII
DV
PurityGreater than 90.0% as determined by(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.
Biological ActivityThe activity of FLT1 was determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surface receptors. The ED50 for this effect is typically 10 ng/ml, corresponding to a specific activity of 100,000IU/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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