Granulocyte Macrophage Colony Stimulating Factor Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14381 Dalton.GM-CSF Porcine Recombinant is purified by proprietary chromatographic techniques.
GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Sterile Filtered White lyophilized (freeze-dried) powder.
GM-CSF Porcine was lyophilized with no additives.
It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Granulocyte Macrophage Colony Stimulating Factor althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Arg-Pro.
Greater than 95.0% as determined by:(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.
The ED50 range= 15-30ng/ml corresponding to a Specific Activity of 33,334-66,667IU/mg, determined by the dose dependent proliferation of TF-1 cells.
NeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.