Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa.The HGF is purified by proprietary chromatographic techniques.
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3and GM-CSFto stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.
Scatter Factor (SF), Hepatopoietin (HPTA), HGF.
Chinese Hamster Ovary Cells.
Sterile Filtered White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.
It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M?-cm H2O not less than 100
Lyophilized Hepatocyte Growth Factor althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid Sequence
Agrees with the sequence of native human HGF.
Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml corresponding to a Specific Activity of 25,000-50,000IU/mg.
NeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.