IGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton. IGFBP5 is purified by proprietary chromatographic techniques.
IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
IGFBP-5, IBP-5, IGF-binding protein 5.
Sterile Filtered White lyophilized (freeze-dried) powder.
IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate PH 3.0.
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized IBP5 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe.
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3
ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs,agricultural or pesticidal products, food additives or household chemicals.
Title:Effects of Dietary Carbohydrate Modificationin Persons with the Metabolic Syndrome- A Transcriptomics Approach in Adipose TissuePublication:Department of Clinical Nutrition, Food and Health Research CentreInstitute of Public Health and Clinical NutritionFaculty of Health SciencesUniversity of Eastern Finland Kuopio 2010Link:http://epublications.uef.fi/pub/urn_isbn_978-952-61-0024-1/urn_isbn_978-952-61-0024-1.pdf