Internalin-A-Other

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Internalin-A

Qty


Total
$221
Catalog #
PRPS-038
Size
Description
Internalin Listeria monocytogenes Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 374 amino acids and having a molecular mass of 42.7kDa. The Internalin-A is fused with an 8 amino acid His tag at C-terminus.
Additional Information
IntroductionInternalins are surface proteins located on Listeria monocytogenes. Internalins exist in two known forms, InlA and InlB. Internalins are used by the bacteria to invade mammalian cells via cadherins transmembrane proteins.The precise role of the Internalin proteins and their invasiveness in vivo is not entirely understood. However, in cultured cells, InlA is needed to facilitate Listeria entry into human epithelial cells. While InlB is required for Listeria internalisation in several other cell types, including hepatocytes, fibroblasts, and epithelioid cells.
SynonymsNULL
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationInternalin-A is lyophilized from a 0.2
SolubilityIt is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100
StabilityLyophilized Internalin-A althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMANENNFLEI
MSETKEIKIV
NIPDNNLKKV
LNKSLNKSEN
SDLTVKDLES
IEYLYGIAEN
ISNIEGLEYC
KNLKILSLQN
NDNSKKENFN
TITDLSPLKY
LKNLVVLDLR
NNKISDLSPL
ENLTNLESLR
LSGNNISNIS
PLNKLESLTT
LTLSYNEITD
ISTISNLKNL
THLALYNNKI
EDISSLKENT
KLQNLSLGFN
KIKDISVLSN
LKNLYDLSLE
ENNIKSIKSL
SN
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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