ITPA Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 215 amino acids (1-194 a.a.) and having a molecular mass of 23.7 kDa. The ITPA is fused to 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
ITPA enzyme catalyzes the pyrophosphohydrolysis of both ITP (inosine triphosphate) and dITP (deoxyinosine triphosphate) to IMP (inosine monophosphate) and diphosphate. IMP is exercised as a substrate for purine nucleotide pathways. IMP is phosphorylated to ITP, and ITPA mediates the concentration of ITP in the cell by changing ITP back to IMP. Defects in ITPA result in ITPA deficiency which is thoµght to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts.