Leptin Human, His-Leptin

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Leptin Human, His

Qty


Total
$65
Catalog #
CYPS-294
Size
Description
Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.The Leptin is purified by proprietary chromatographic techniques.
Additional Information
IntroductionLeptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells.
SynonymsOB Protein, Obesity Protein, OBS, Obesity factor.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.
SolubilityThe lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
StabilityLyophilized Leptin althoµgh stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.Please avoid freeze-thaw cycles.
PurityGreater than 90.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Applications1. Positive control for Western blot.2. Antibody production. 3. Protein assay.
Background

N/A

Protocol

N/A

MSDS
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