M CSF Human, Baculovirus-Colony Stimulating Factor

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M CSF Human, Baculovirus

Qty


Total
$65
Catalog #
CYPS-644
Size
Description
Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, glycosylated, polypeptide chain containing 2 x 149 amino acids and having a total molecular mass of 42 kDa.MCSF is purified by proprietary chromatographic techniques.
Additional Information
IntroductionGranulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
SynonymsCSF-1, Lanimostim, MCSF, MGC31930, M-CSF, Macrophage colony-stimulating factor 1, CSF1.
SourceBaculovirus infected Silkworm.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe lyophilized protein (1mg/ml) was lyophilized with 20mM phosphate buffer, 1% HSA and 3% manntiol.
SolubilityIt is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Macrophage Colony Stimulating Factor althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Amino Acid SequenceEEVSEYCSHM
IGSGHLQSLQ
RLIDSQMETS
CQITFEFVDQ
EQLKDPVCYL
KKAFLLVQDI
MEDTMRFRDN
TPNAIAIVQL
QELSLRLKSC
FTKDYEEHDK
ACVRTFYETP
LQLLEKVKNV
FNETKNLLDK
DWNIFSKNCN
NSFAECSSQ.
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe ED50,calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 3ng/ml, corresponding to a specific activity of less than 333,333.33units/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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