MIP 1b Human-MIP

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MIP 1b Human

Qty


Total
$65
Catalog #
CHPS-283
Size
Description
Macrophage Inflammatory Protein-1 beta Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7620 Dalton. The CCL4 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionMacrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b inducesynthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.
SynonymsSmall inducible cytokine A4, CCL4, Macrophage inflammatory protein 1-beta, MIP-1- beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, H400, SIS-gamma, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein,
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
SolubilityIt is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized MIP-1b althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceThe
sequence
of
the
first
five
N-terminal
amino
acids
was
determined
and
was
found
to
be,
Ala-Pro-Met-Gly-Ser.
PurityGreater than 99.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
ReferencesTitle: CONFUSION BETWEEN CCL4 AND CCL4L1: AN EXAMPLE TO BEAR IN MIND WHEN USING THIRD-PARTY REAGENTS.Publication: Inmunologia Vol. 26 / Num 1/ Enero-Marzo 2007: 51-54 (Spanish).Link: http://revista.inmunologia.org/Upload/Articles/7/5/756.pdf
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