MYL5 (1-173 a.a.) Human-Myosin Light Chain

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MYL5 (1-173 a.a.) Human

Qty


Total
$65
Catalog #
PRPS-950
Size
Description
MYL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (1-173 a.a.) and having a molecular mass of 22.1kDa.MYL5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionMyosin regulatory light chain 5 (MYL5) is a hexameric ATPase cellular motor protein. Myosin is comprised of 2 heavy chains, 2 nonphosphorylatable alkali light chains, and 2 phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in the fetal muscle and in the adult retina, cerebellum, and basal ganglia. The reconstitution of myosin with MYL5 or alkali light chain increases filament velocity to intermediate rates, and the re-addition of both classes of light chains fully reinstates the original sliding pace.
SynonymsMyosin light chain 5, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2, MYLC2, MyLC-2, MYL5.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationMYL5 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl,1mM DTT and 0.1mM PMSF.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSHMASRKT
KKKEGGALRA
QRASSNVFSN
FEQTQIQEFK
EAFTLMDQNR
DGFIDKEDLK
DTYASLGKTN
VKDDELDAML
KEASGPINFT
MFLNLFGEKL
SGTDAEETIL
NAFKMLDPDG
KGKINKEYIK
RLLMSQADKM
TAEEVDQMFQ
FASIDVAGNLDYKALSYVIT
HGEEKEE.
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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