NANOG-TAT Human-NANOG

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NANOG-TAT Human

Qty


Total
$65
Catalog #
PRPS-097
Size
Description
NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 305 amino acids and having a molecular mass of 34.6kDa.The NANOG is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by proprietary chromatographic techniques.
Additional Information
IntroductionNANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
SynonymsNANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationNANOG was lyophilized after extensive dialysis against PBS.
SolubilityIt is recommended to reconstitute the lyophilized NANOG in sterile PBS not less than 100
StabilityLyophilized NANOG althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NANOG should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Amino Acid SequenceThe
sequence
of
the
first
five
N-terminal
amino
acids
was
determined
and
was
found
to
be
Met-Gly-Arg-Lys-Lys.
PurityGreater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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