NDK E.Coli-Protein Kinases

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NDK E.Coli

Qty


Total
$65
Catalog #
PKPS-041
Size
Description
NDK E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-143 a.a.) and having a molecular mass of 18kDa.NDK is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionNucleoside diphosphate kinase (Ndk) is an enzyme which catalyzes the exchange of phosphate groups between different nucleoside diphosphates. Ndk’s activity sustains equilibrium between the concentrations of various nucleoside triphosphates for example, when GTP is produced in the citric acid (Krebs) cycle is converted to ATP. Ndk has a key role in the synthesis of nucleoside triphosphates other than ATP.
SynonymsNucleoside diphosphate kinase, NDK, NDP kinase, Nucleoside-2-P kinase, b2518, JW2502.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationNDK protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSHMAIERT
FSIIKPNAVA
KNVIGNIFAR
FEAAGFKIVG
TKMLHLTVEQ
ARGFYAEHDG
KPFFDGLVEF
MTSGPIVVSV
LEGENAVQRH
RDLLGATNPA
NALAGTLRAD
YADSLTENGT
HGSDSVESAA
REIAYFFGEG
EVCPRTR.
PurityGreater than 95% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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