NMM Human-Myosin Light Chain

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NMM Human

Qty


Total
$65
Catalog #
PRPS-373
Size
Description
Non-Muscle Myosin-II Regulatory Light Chain Human Recombinant full length expressed in E.coli.The NMM is purified by proprietary chromatographic techniques.
Additional Information
IntroductionNon muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daµghter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca +2 Calmodulin regulated light chain kinase (MLCK).
SynonymsNon-Muscle Myosin-II Regulatory Light Chain, NMM.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered clear solution.
FormulationNMM protein at 0.2mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.
StabilityStore vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles.
Amino Acid SequenceMSSKKAKTKT
TKKRPQRATS
NVFAMFDQSQ
IQEFKEAFNM
IDQNRDGFID
KEDLHDMLAS
LGKNPTDAYL
DAMMNEAPGP
INFTMFLTMF
GEKLNGTDPE
DVIRNAFACF
DEEATGTIQE
DYLRELLTTM
GDRFTDEEVD
ELYREAPIDK
KGNFNYIEFT
RILKHGAKDK
DD.
PurityGreater than 95.0% as determined by SDS-PAGE.
Biological ActivityAssayed for phosphorilation by MLCK.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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