proNT3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4kDa. The proNT3 is purified by proprietary chromatographic techniques.
proNT-3 (the precursor form of neurotrophin-3) functions throµgh p75NTR and sortilin and induces neuronal apoptosis. In contrast, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. Since axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also has an important role in the mechanism of retrograde signaling.
Precursor Form Neurotrophin-3, proNT-3.
Sterile Filtered White lyophilized (freeze-dried) powder.
proNT3 was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0 and 300mM NaCl.
It is recommended to reconstitute the lyophilized proNT3 in sterile 18M-cm H2O not less than 100
Lyophilized proNT3 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution proNT3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln.
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
NeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.