Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue- Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have sµggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also sµggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drµgs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drµgs.Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Recombinant Human Resistin was lyophilized from a concentrated (1mg/ml) solution containing 0.03% NaHCO3.
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Greater than 98% as determined by SDS-PAGE.
The biological activity was evidenced by the Resistin ability to prevent insulin-stimulated uptake of deoxy glucose in several cell lines at 10 ng/ml concentration corresponding to a Specific Activity of 100,000IU/mg.
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Two-step procedure using size exclusion chromatography before and after refolding.
The amino acid sequence of the Resistin Human Recombinant ishomologous to the amino acid sequence of the Resistin Human without signal sequence.