rGFP-Other

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rGFP

Qty


Total
$65
Catalog #
PRPS-694
Size
Description
rGFP Aequorea victoria produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-238 a.a.) and having a molecular mass of 26.8 kDa.rGFP is purified by proprietary chromatographic techniques.
Additional Information
IntroductionrGFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. rGFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
SynonymsGreen fluorescent protein, GFP.
SourceEscherichia Coli.
Physical AppearanceSterile filtered yellowish solution.
FormulationThe Green Fluorescent Protein solution contains 20mM Tris-HCl, pH-8 and 10% Glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMSKGEELFTG
VVPILVELDG
DVNGHKFSVS
GEGEGDATYG
KLTLKFICTT
GKLPVPWPTL
VTTFSYGVQC
FSRYPDHMKQ
HDFFKSAMPEGYVQERTIFF
KDDGNYKTRA
EVKFEGDTLV
NRIELKGIDF
KEDGNILGHK
LEYNYNSHNV
YIMADKQKNG
IKVNFKIRHN
IEDGSVQLADHYQQNTPIGD
GPVLLPDNHY
LSTQSALSKD
PNEKRDHMVL
LEFVTAAGIT
HGMD
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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