Rhodanese Human-Transferase

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Rhodanese Human

Qty


Total
$65
Catalog #
ENPS-466
Size
Description
Recombinant Human Rhodanese produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a) and having a molecular mass of 35.6 kDa. Rhodanese is fused to a 20 amino acid His-Tag at N-terminus and purified by conventional chromatography techniques.
Additional Information
IntroductionRhodanese is a mitochondrial matrix enzyme that is encoded by the nucleus. Rhodanese is involved in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. Rhodanese catalyzes the chemical reaction of thiosulfate & cyanide to sulfite & thiocyanate (detoxification). Rhodanese is part of the transferase family of proteins. Rhodanese includes two highly conservative domains, identified as rhodanese homology domains. In mammals, the majority of cyanide is converted to thiocyanate. Rhodanese has weak mercaptopyruvate sulfurtransferase activity.
SynonymsEC 2.8.1.1, TST, MGC19578, RDS, Thiosulfate sulfurtransferase, Rhodanese.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe Rhodanese protein solution contains 20mM Tris-HCl, pH-8 and 10% Glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MVHQVLYRAL
VSTKWLAESI
RTGKLGPGLR
VLDASWYSPG
TREARKEYLE
RHVPGASFFD
IEECRDTASP
YEMMLPSEAG
FAEYVGRLGI
SNHTHVVVYD
GEHLGSFYAP
RVWWMFRVFG
HRTVSVLNGG
FRNWLKEGHP
VTSEPSRPEP
AVFKATLDRS
LLKTYEQVLE
NLESKRFQLV
DSRSQGRFLG
TEPEPDAVGL
DSGHIRGAVN
MP
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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