SEC13 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 342 amino acids (1-322 a.a.) and having a molecular mass of 37.7kDa. The SEC13 is purified by proprietary chromatographic techniques.
SEC13 is a member of the SEC13 family of WD-repeat proteins. SEC13 is a constituent of the endoplasmic reticulum and the nuclear pore complex. SEC13 is similar to the yeast SEC13 protein that is essential for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. SEC13 interacts with SEC31A and SEC31B. SEC31A colocalizes with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function.
Protein SEC13 homolog, SEC13-like protein 1, SEC13-related protein, SEC13, D3S1231E, SEC13L1, SEC13R, npp-20.
Sterile Filtered colorless solution.
The SEC13 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 100mM NaCl.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.