TGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa. The TGFB1 is purified by proprietary chromatographic techniques.
Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a concentrated (1mg/ml) solution containing 10mM HCl & 50µg BSA per mg/ml of protein.
It is recommended to reconstitute the lyophilized TGFB1 in sterile 5mM HCl & 50µg/ml BSA at a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized TGFB1 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
The ED50, as measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells is typically 0.1-0.15ng/ml.
NeoScientific's products are furnished forLABORATORY RESEARCHUSEONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
1.Title:Imatinib attenuates skeletal muscle dystrophy in mdx mice .Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 Aµgust 2009 The FASEB Journal vol. 23 no. 8 2539-2548 .Link:http://www.fasebj.org/content/23/8/2539.full2.Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins.Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal.pone.0027152Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027152