THTPA Human-Phosphatase

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THTPA Human

Qty


Total
$65
Catalog #
ENPS-256
Size
Description
Recombinant Human THTPA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-230 a.a.) and having a molecular mass of 27.7 kDa. THTPA is fused to a 20 amino acid His-Tag at N-Terminus and purified by conventional chromatography techniques.
Additional Information
IntroductionTHTPA enzyme is part of the THTPase family. THTPA is localized to the cytoplasm and expressed at small quantities in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the main form of thiamine within the cell. THTPA occurs as a monomer and is activated at an optimal pH of 8.5.
SynonymsMGC2652, THTP, THTPASE.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe THTPA 1mg/ml protein solution contains 20mM Tris-HCL buffer, pH-8, 1mM DTT and 10% Glycerol.
StabilityStore THTPA at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MAQGLIEVER
KFLPGPGTEE
RLQELGGTLE
YRVTFRDTYY
DTPELSLMQA
DHWLRRREDS
GWELKCPGAA
GVLGPHTEYK
ELTAEPTIVA
QLCKVLRADG
LGAGDVAAVL
GPLGLQEVAS
FVTKRSAWKL
VLLGADEEEP
QLRVDLDTAD
FGYAVGEVEA
LVHEEAEVPT
ALEKIHRLSS
MLGVPAQETA
PAKLIVYLQR
FRPQDYQRLL
EV
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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