TWF1 Human-Other

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TWF1 Human

Qty


Total
$65
Catalog #
PRPS-1010
Size
Description
TWF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 277 amino acids (1-252 a.a.) and having a molecular mass of 31.5kDa. TWF1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionTwinfilin (TWF1) is a ubiquitous actin-monomer-binding protein which is composed of two ADF-homology domains. Twinfilin forms a 1:1 complex with ADP-actin-monomers, prevents nucleotide exchange on actin monomers and blocks assembly of the monomer into filaments. TWF1 is composed of 2 ADF/cofilin-like (ADF-H) domains joined by a short linker region and followed by a C-terminal tail of approximately 20 residues. The 2 ADF-H domains are approximately 20% homologous to ADF/cofilin and to each other. TWF1 protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures might be regulated by the small GTPase RAC1.
SynonymsTwinfilin-1, Protein A6, Protein tyrosine kinase 9, TWF1, PTK9.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationTWF1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSHMMLYAA
TRATLKKEFG
GGHIKDEVFG
TVKEDVSLHG
YKKYLLSQSS
PAPLTAAEEE
LRQIKINEVQ
TDVGVDTKHQ
TLQGVAFPIS
REAFQALEKL
NNRQLNYVQL
EIDIKNEIII
LANTTNTELK
DLPKRIPKDS
ARYHFFLYKH
SHEGDYLESI
VFIYSMPGYT
CSIRERMLYS
SCKSRLLEIV
ERQLQMDVIR
KIEIDNGDEL
TA
PurityGreater than 80% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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