TYW5 Human-Other

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TYW5 Human

Qty


Total
$65
Catalog #
PRPS-1322
Size
Description
TYW5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (1-315 a.a.) and having a molecular mass of 38.9kDa.TYW5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductiontRNA wybutosine-synthesizing protein 5 (TYW5) functions as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position closest to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine observed in higher eukaryotes.
SynonymstRNA wybutosine-synthesizing protein 5, hTYW5, TYW5, C2orf60.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationTYW5 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSMAGQHLP
VPRLEGVSRE
QFMQHLYPQR
KPLVLEGIDL
GPCTSKWTVD
YLSQVGGKKE
VKIHVAAVAQ
MDFISKNFVY
RTLPFDQLVQ
RAAEEKHKEF
FVSEDEKYYL
RSLGEDPRKD
VADIRKQFPL
LKGDIKFPEF
FKEEQFFSSV
FRISSPGLQL
WTHYDVMDNL
LIQVTGKKRV
VLFSPRDAQY
LYLKGTKSEV
LNIDNPDLAK
YP
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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