Ubiquitin-Conjµgating Enzyme E2I Human Recombinant produced in E.coli is a 19.5 kDa protein containing 171 amino acids.The UBE2I protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
Human Ubquitin Conjµgating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjµgation of SUMO to a variety of target proteins. SUMO conjµgation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjµgation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase.
SUMO-conjµgating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjµgating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.
Sterile Filtered white lyophilized powder.
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100
Lyophilized UBE2I althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2I should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.