UFM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids (1-83 a.a) and having a molecular mass of 11.1kDa. The UFM1 is purified by proprietary chromatographic techniques.
UFM1 is a ubiquitin-like protein which is conjµgated to target proteins by E1-like activating enzyme UBA5 and E2-like conjµgating enzyme UFC1 in a manner equivalent to ubiquitylation. UFM1 localizes principally to the nucleus, but is also present in diffuse amounts in the cytoplasm. UFM1 is expressed in a variety of tissues, including kidney, brain, heart, liver and lung.
The UFM1 solution (1 mg/ml) 20mM Tris buffer (pH 8.0) and 10% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.