UMPS Human-Synthetase

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UMPS Human

Qty


Total
$65
Catalog #
ENPS-670
Size
Description
UMPS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 500 amino acids (1-480 a.a) and having a molecular mass of 54.3kDa.UMPS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionUridine 5'-monophosphate synthase (UMPS), is a bifunctional enzyme that catalyzes the ultimate two steps of the de novo pyrimidine biosynthetic pathway. UMPS in eukaryotes links the orotate phosphoribosyltransferase and the orotidine-5’-monophosphate (OMP) decarboxylase activities into a single protein. The harmony of these 2 enzymes is assumed to be stabilized the catalytic centers as a result of the low molar concentration of the protein in mammalian cells.mutations in this gene are the reason of inherited orotic aciduria disease.
SynonymsOPRT, Uridine 5'-monophosphate synthase, UMP synthase, Orotate phosphoribosyltransferase , OPRT, OPRTase, Orotidine 5'-phosphate decarboxylase , ODC, OMPdecase.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered clear solution.
FormulationThe UMPS solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea and 20% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MAVARAALGP
LVTGLYDVQA
FKFGDFVLKS
GLSSPIYIDL
RGIVSRPRLL
SQVADILFQT
AQNAGISFDT
VCGVPYTALP
LATVICSTNQ
IPMLIRRKET
KDYGTKRLVE
GTINPGETCL
IIEDVVTSGS
SVLETVEVLQ
KEGLKVTDAI
VLLDREQGGK
DKLQAHGIRL
HSVCTLSKML
EILEQQKKVD
AETVGRVKRF
IQENVFVAAN
HN
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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