Vimentin Human-Vimentin

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Vimentin Human

Qty


Total
$65
Catalog #
PRPS-316
Size
Description
Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 Dalton (calculated from sequence), 57,000 Dalton (determined by SDS gelelctrophoresis).
Additional Information
IntroductionVimentin expression in human malignant glioma cells depends on cellular density, algorithms of drµg delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.
SynonymsVimentin, Vim, FLJ36605.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe protein (1mg/ml) was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride.
SolubilityIt is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Vimentin althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid Sequencemstrsvssss
yrrmfggpgt
asrpsssrsy
vttstrtysl
gsalrpstsr
slyasspggv
yatrssavrl
rssvpgvrll
qdsvdfslad
aintefkntr
tnekvelqel
ndrfanyidk
vrfleqqnki
llaeleqlkg
qgksrlgdly
eeemrelrrq
vdqltndkar
veverdnlae
dimrlreklq
eemlqreeae
ntlqsfrqdv
dnaslarldl
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ia
PurityGreater than 95.0% as determined by SDS-gelelctrophoresis.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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