VRK3 Human-Other

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VRK3 Human

Qty


Total
$65
Catalog #
PRPS-1137
Size
Description
VRK3 Human Recombinant produced in E. coli is a single polypeptide chain containing 497 amino acids (1-474) and having a molecular mass of 55.3 kDa.VRK3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionVRK3 is a member of the protein kinase superfamily. The VRK3 gene has replacements at a number of residues within the ATP binding motifs which in other kinases were proved to be required for catalysis in both human and mouse. In vitro assays show that VRK3 has no phosphorylation activity but probably holds its substrate binding capability. VRK3 is universally expressed in human tissues and its protein is restricts to the nucleus.
SynonymsSerine/threonine-protein pseudokinase VRK3, vaccinia related kinase 3, inactive serine/threonine-protein kinase VRK3.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe VRK3 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 40% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSMISFCPD
CGKSIQAAFK
FCPYCGNSLP
VEEHVGSQTF
VNPHVSSFQG
SKRGLNSSFE
TSPKKVKWSS
TVTSPRLSLF
SDGDSSESED
TLSSSERSKG
SGSRPPTPKS
SPQKTRKSPQ
VTRGSPQKTS
CSPQKTRQSP
QTLKRSRVTT
SLEALPTGTV
LTDKSGRQWK
LKSFQTRDNQ
GILYEAAPTS
TLTCDSGPQK
QKFSLKLDAK
DG
PurityGreater than 85% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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