WIF1 produced in Hi-5 cells is a single, glycosylated polypeptide chain containing 369 amino acids (29-379 a.a.) and having a molecular mass of 40.5 kDa, (48kDa on SDS-PAGE). WIF1 is fused to 16 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
WIF1 binds to wnt proteins and inhibits their activities. WIF1 plays a role in mesoderm segmentation. WNT proteins are extracellular signaling molecules that take part in the control of embryonic development & cancer. WIF1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. WIF1 takes part in mesoderm segmentation. WIF1 protein is found to be present in fish, amphibia and mammals. WIF1 is a recurrent target in human salivary gland oncogenesis. Downregulation of WIF1 takes part in the development and progression of pleomorphic adenomas. WIF1 is a tumor suppressor, specifically in nonfunctioning pituitary tumors.
WIF1, WIF-1, Wnt inhibitory factor 1.
Sterile filtered colorless solution.
The WIF1 protein solution contains 1X PBS pH 7.4, 10% glycerol and 1mM PMSF.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.